‘Another set of fingers’

first_imgAn interdisciplinary group of leading Harvard geneticists and stem cell researchers has found a new genetic aspect of cell reprogramming that may ultimately help in the fine-tuning of induced pluripotent stem cells (iPS) into specific cell types.The researchers, who have affiliations with the Harvard Stem Cell Institute (HSCI), the Broad Institute of Harvard and MIT, Harvard’s Department of Stem Cell and Regenerative Biology (SCRB), Children’s Hospital Boston, and Beth Israel Deaconess Medical Center (BIDMC), have identified a set of genetic elements never before known to be involved in cellular reprogramming.According to John Rinn, a corresponding author of the study who is affiliated with HSCI, SCRB, the Broad, and BIDMC, “this is the first time that this new type of gene has been implicated in the reprogramming process. In reprogramming it’s important to find as many routes to reprogramming as possible. This finding gives us another set of fingers to play on the genomic piano that controls the ‘music’ of cellular reprogramming.”“This is a group of RNAs that come from the dark region of the genome,” Rinn said. “We don’t know everything they do, but they clearly are involved in the reprogramming process.”“We know reprogramming resets the genome [of the reprogrammed cell] globally,” said George Daley of HSCI and Children’s Hospital and, along with Rinn, one of the two senior authors of a paper published online by Nature Genetics. “What we’ve learned by working with John is that lincRNAs play essential roles in the reprogramming process. We’ve even identified one lincRNA that enhances reprogramming, which is tantalizing evidence that we can make reprogramming more efficient by harnessing lincRNAs.”Added Daley, a biologist, “our groups came together to answer a question that neither group could answer alone.”The new finding follows by less than two months the discovery by HSCI’s Derrick Rossi and colleagues that skin cells could be returned to an embryonic stem cell-like state using specially engineered synthetic mRNAs, eliminating the need to use viruses that can induce cancers to develop.Rossi’s discovery turned the cell reprogramming field on its head, and was considered so significant that HSCI co-director Doug Melton announced that the HSCI iPS core, which produces reprogrammed cells for researchers, would immediately switch to using the method developed by Rossi.The new findings by Rinn, Daley, and colleagues may help explain why Rossi’s method works — because RNAs appear to be part of the natural cell programming process — and may also be used to refine the Rossi reprogramming method, Rinn said. “What we think we’ve done is, [we’ve found] another way to throw the reprogramming switches,” Rinn said.The road to this latest discovery began when Rinn first arrived at Harvard and the Broad in January 2009. He, Broad director Eric Lander, and Mitch Guttman, who are also authors on the Nature Genetics paper, discovered a group of 5,000 lincRNAs. “Then we worked with George (Daley) to figure out their relevance, if any, in reprogramming,” Rinn said.Like much of scientific discovery, the latest finding was serendipitous. As Rinn explains it:“We started out looking for something else: We wanted to know what happens when you program skin cells that originate in different parts of the body into stem cells. George and I were on a committee meeting together with a student who didn’t know the anatomic origin of the skin cells used to make stem cells. A light bulb went off that it may be interesting to make stem cells from skin cells originating all around the body and see if they ‘remember’ where they came from. Sabine Loewer, the lead author on the paper, immediately jumped at the idea as well — which was very brave for such a risky project.“It turned out they didn’t matter where the cells came from,” Rinn said, “which is in itself an important finding. But in the process we found that hundreds of lincRNA genes were turned on and off during the reprogramming, so we focused on the ones that turned on, to see if they were part of the pluripotency process, and they were.”last_img read more

Pelicans’ Julius Randle expected to decline player option, report says

first_imgIt appears like Julius Randle will test free agency.The Pelicans big man is expected to decline his $9 million player option this offseason, according to a report from The Athletic, which cites unidentified league sources. They are also expected to select Duke star Zion Williamson with the No. 1 pick on June 20.Williamson has drawn comparisons to Randle, who is taller by two inches at 6-9 but is also more than 30 lbs lighter. It’s unclear how New Orleans will proceed, as it will have two undersized, athletic, left-handed forwards on the roster if they keep both.Nevertheless, New Orleans has plenty of options. Was very important for Randle to get another shot in free agency next summer if he wants it. https://t.co/wPXMzJJOXf— Zach Lowe (@ZachLowe_NBA) July 2, 2018″ target=”_blank”> report last year.The Pelicans agreed to send Anthony Davis to the Lakers on Saturday in exchange for Brandon Ingram, Lonzo Ball, Josh Hart and three first-round draft picks, including the No. 4 pick in the 2019 draft. NBA free agency rumors: Kings prioritizing center, but unlikely to pursue Nikola Vuceviccenter_img NBA trade rumors: Nuggets explored pairing Anthony Davis with Nikola Jokic Pelicans‘ Julius Randle is not picking up his $9M player option for next season and will enter unrestricted free agency, league sources tell @TheAthleticNBA @Stadium. Randle is coming off his best NBA season (21.4 PPG, 8.7 RPG, 34.4 percent from 3-point range on 195 attempts).— Shams Charania (@ShamsCharania) June 17, 2019The 24-year-old is coming off a career year. He averaged 21.4 points and 8.7 rebounds per game with New Orleans this past season.Randle left the Lakers in free agency after 2017-18. His two-year, $18 million deal with the Pelicans was meant to give him another shot at free agency in the summer of 2019 after he proved himself in New Orleans, according to an ESPN Related Newslast_img read more

Kyrie Irving posts lengthy social media response to angry Celtics fans

first_imgKyrie Irving heard the Celtics’ fans harsh words for him even though he wasn’t inside Boston’s TD Garden on Wednesday night. He had a few things to say in response.The injured Nets guard did not make the trip to the place he called home the previous two seasons, and he was skewered in absentia. Chants of “Kyrie sucks” pierced the air early in the night as fans voiced their opinions of the point guard who left Beantown for Brooklyn in the offseason. Irving wasn’t about to let that go. His response on Instagram centered on fans and media lacking perspective about sports in general, plus some of his personal struggles through the years. “This Game of Sports entertainment matters more than someone’s mental health and well being right?” Irving wrote. Kyrie Irving just posted a long IG story: pic.twitter.com/WiryDi1m4b— Boston.com Celtics News (@BDCCeltics) November 28, 2019MORE: Jarrett Allen shoots down Kyrie mood swings rumorIrving has missed the Nets’ past six games with a shoulder ailment. Brooklyn lost 121-110 on Wednesday. Irving’s replacement in Boston, Kemba Walker, scored a game-high 39 points in his return from a neck injury.Irving could return to action Friday when the Celtics travel to Barclays Center for a return visit.last_img read more

Busy Pajama Day

first_imgBy Kathy MieleSteven walked into the room and had a concerned look on his face. It was 2 o’clock in the afternoon and I was still in my pajamas.“Are you feeling alright?” he asked.“Can you hold on for a minute?” I said to my sister, then turned my head so Steven could see that I had my phone’s headset on. “Hey, you’re home early for a Saturday,” I said to him.“I’m just stopping in for a minute. I have two more appointments before I’m really home,” he said.“Hey, Liz can I call you right back? Steven just stopped in for a minute.” I clicked my headset off and turned back to Steven. “I’m sorry. What did you say when you first walked in?”“I asked if you were feeling OK.”“Yeah, why?”“You’re still in your pajamas and bathrobe,” he said.I put down the shorts I’d been sewing a button on. “No, actually I’ve had a really busy day,” I said. “I’ve baked a batch of brownies, I’m on my third load of wash, and I’ve got a pot of sauce simmering for dinner.”I cinched my robe tighter as I headed for the laundry room to switch a load out of the washer and into the dryer. “Right now I’m working on some clothes that needed mending.”“Oh,” Steven sounded happy. “Did you get a chance to sew that missing button on my shirt?”“Done,” I answered, pointing to the pile of finished pieces already on top of the dryer.Reloading the dryer, I hit the start button and headed for the kitchen. “Do you want me to make you some deviled eggs?” I asked. “I hard-boiled a dozen earlier this morning.”“Sure, that sounds great,” Steven said as he followed me into the kitchen.“Careful, I just washed the floor. It’s not completely dry yet,” I called as he stayed in the doorway and I tiptoed in to get the eggs started.“Wow, you have been busy,” he said leaning against the doorframe.“I’ve gotten so much done. I love pajama day!” I said, cracking the shell of the first egg. “How’s your day going?” I asked.“Busy,” he said. “But not as busy as yours.”I had to laugh. “I’ve still got a few more things I want to get done before I get showered and dressed. But at the rate I’m going, if it gets any closer to dinnertime before I’m finished I might as well get showered and put on a fresh set of jammies!”I handed him a plate with a few deviled eggs and sat next to him at the dining room table while he ate.Steven shrugged his shoulders. “Hey, who’s going to know, right?”Just then the doorbell rang. I looked around the corner and saw a neighbor standing at our front door. I leaned back against my chair, trying not to be seen. “Can you get that for me?” I asked.Steven looked at me. “What’s wrong?” he asked.“Are you kidding?” I waved my hand in front of me. “I’m in my pajamas! They’re going to think I’m some sort of sloth, just hanging around all day.”“You could always explain what pajama day means to you,” he said.“Very funny,” I said as I hid in the kitchen while Steven got up to get the door.“By the way,” I whispered. “I’m not home, OK?”last_img read more

HALO FARMS’ HOMEBRED DANZING CANDY CRUISES TO TWO LENGTH WIN IN GRADE II, $400,000 SAN FELIPE STAKES; SMITH & SISE TEAM FOR 1 1/16 MILES WIN IN 1:43.04

first_imgARCADIA, Calif. (March 12, 2016)–Halo Farms’ front-running Danzing Candy cruised to an impressive two length win under Mike Smith in Saturday’s Grade II, $400,000 San Felipe Stakes at Santa Anita. Trained by Clifford Sise, the lightly raced Kentucky-bred colt by Twirling Candy got 1 1/16 miles in 1:43.04 and picked up 50 Kentucky Derby qualifying points, assuring him of a berth in the Run for the Roses on May 7.“He warmed up great, he was on his toes,” said Smith. “Our game plan was to put him on the lead unless he didn’t jump out of there well. We didn’t want to experiment…I left there very aggressive and he didn’t get away with anything (slow fractions). If you go 22 on this track today, you’re smokin’. And for him to hold off the caliber of horses he held off, was very impressive.”Bred by Ted Aroney’s Halo Farms and owned by Halo Farms and Jim and Diane Bashor, Danzing Candy paid $13.00, $5.20 and $3.40. A maiden special weight winner two starts back on Dec. 26, Danzing Candy was a 5 ¾ length allowance winner here on Feb. 4 and thus picked up his third win from four starts. With the winner’s share of $240,000, he increased his earnings to $308,650.“I expected him to be on the lead,” said Sise. “We didn’t want to experiment in this race. Mike just said, ‘I’ll let him come out the first few jumps and if he’s there, he’s there. If somebody sends, he’ll sit second.“He (broke) much better today. He’s good now. We’ll stay for the Santa Anita Derby (Grade I, $1 million at 1 1/8 miles April 9). He’s three for three on this track. Why would we change now? I don’t see any reason to, but you never know. You’ve got to leave that up to the owners.”Ridden by Gary Stevens, favored Mor Spirit appeared to get a bit rank around the Club House turn, but settled readily down the backside when next to last going past the half mile pole. With a cue from Stevens, he picked it up between horses around the far turn, rallied well, while within himself for second money and galloped out on terms with the winner past the wire.Off at 8-5 in a field of six Derby hopefuls, Mor Spirit paid $3.40 and $2.20.“I’m very happy, because he was way too keen in the early part of the race, he was really fresh…” said Stevens. “He was super sharp. As I was coming into the lane, I knew I wasn’t going to catch the winner, but I knew we had to get some (Kentucky Derby) points. I like where we’re sitting for the Santa Anita Derby. I lost a battle today, but I like our position. Bob (Baffert) was happy, so I’m happy…He’ll settle a little better for me in the Santa Anita Derby.Exaggerator, who is trained by Keith Desormeaux and ridden by his brother, Kent, was content to lag early and picked it up in eye-catching fashion heading into the far turn as he skimmed the rail, but he flattened out late, finishing three quarters of length behind Mor Spirit.The second wagering choice at 2-1, Exaggerator paid $2.40 to show.With the winner getting 50 Kentucky Derby qualifying points, the second, third and fourth place finishers earned 20, 10 and five points respectively.Danzing Candy set fractions of 22.96, 46.11, 1:11.04 and 1:36.38 over a main track that although was listed as fast, had been dulled by heavy afternoon rains on Friday. 1-2-3 FINISHERS HEADED TO GRADE I, $1 MILLION SANTA ANITA DERBY ON APRIL 9last_img read more

France v Croatia: 3 key World Cup final battles

first_img0Shares0000Creative forces: Croatia’s Luka Modric (right) and Antoine Griezmann of France could hold the key to the World Cup final © AFP / Pierre-Philippe MARCOU, Gabriel BOUYSMOSCOW, Russian Federation, Jul 14 – France are favourites to win the World Cup for a second time in Moscow on Sunday when they take on a Croatia side forced to go through three periods of extra-time to make their first ever final.By contrast, Didier Deschamps’s side have seen off Argentina, Uruguay and Belgium, all in 90 minutes, and enjoyed a day’s extra rest to prepare after their semi-final on Tuesday as they look to make amends for defeat in the Euro 2016 final to Portugal. As the smallest country to reach a World Cup final for 68 years, Croatia cannot call on the same resources as the French but have shown incredible mental strength to battle back from falling behind in each of their three knockout games.AFP Sport looks at three key battles that could decide who are crowned champions of the world:Luka Modric v Paul PogbaPaul Pogba has done his share of defending to help France to the World Cup final © AFP / Adrian DENNISModric has been widely hailed as the world’s best midfielder for his performances in Russia and has emerged as a Ballon d’Or contender after also winning his fourth Champions League in five years at Real Madrid.That is a status Pogba aspires to, but the one-time world’s most expensive player has sacrificed some of his creative talents for the good of the team in Russia.Manchester United’s Pogba has formed a formidable partnership with N’Golo Kante at the heart of the French midfield that has helped snuff out the threat of Lionel Messi, Kevin De Bruyne and Eden Hazard.Should Pogba and Kante also prevent Modric from taking control of the midfield as he did despite tired legs in the second half of Croatia’s semi-final against England, it will take France a long way towards a first World Cup in 20 years.Mario Mandzukic v Raphael VaraneMario Mandzukic scored Croatia’s semi-final winner against England © AFP / Yuri CORTEZMandzukic often saves his best for the big occasion, most recently shrugging off a knee injury to strike the winner against England in the semi-final.The Juventus forward also has a great record when facing Varane. Mandzukic scored a stunning overhead kick in the 2017 Champions League final, netted twice as the Italians beat Real Madrid 3-1 at the Santiago Bernabeu in April and grabbed the winner for Atletico Madrid in the 2014 Spanish Super Cup.Mandzukic will be used as a focal point to try and bring Modric, Ivan Rakitic and Ivan Perisic into the game, but Varane has arguably been the best centre-back at the World Cup and has the speed, height and power to nullify Croatia’s biggest goal threat.Marcelo Brozovic v Antoine GriezmannDifference maker: Antoine Griezmann has scored three goals and set up two more in Russia © AFP / FRANCK FIFERecalled by Zlatko Dalic for the semi-final, Inter Milan midfielder Brozovic freed up Modric and Rakitic from defensive duties at the base of the midfield and always provided an out ball for his centre-backs as Croatia dominated possession.Griezmann has not shone as brightly as at Euro 2016, when he won the Golden Boot. His three goals have come from two penalties and a goalkeeping mistake by Uruguay’s Fernando Muslera.However, the Atletico Madrid star has improved as the tournament has progressed and has been France’s main playmaker, providing the assists for Varane’s and Samuel Umtiti’s goals in the quarter-finals and semi-finals.Griezmann’s energy and intelligence will see him find space to feed Giroud and Kylian Mbappe if Croatia’s energy levels run low. It’ll be Brozovic’s job to make sure he does not.0Shares0000(Visited 1 times, 1 visits today)last_img read more

How Liverpool could line up next season with Mario Gotze and more signings

first_img Attacking midfield: Philippe Coutinho Central midfield: Piotr Zielinski 11 11 Right back: Nathaniel Clyne 11 11 Centre back: Mamadou Sakho 11 11 11 11 Attacking midfield: Roberto Firmino 11 Goalkeeper: Marc-Andre ter Stegen – Click the right arrow above to see how Liverpool could look next season… 11 11 Liverpool might still be on the fringes of a top four battle but that isn’t stopping them planning next season’s transfers.Reds boss Jurgen Klopp has already lined up a deal to bring in Schalke’s Joel Matip but now rumours are circulating he’ll be doing all he can to land Bayern Munich star Mario Gotze.There is plenty more speculation too with the German’s defence and midfield needing some tinkering over the summer.But how might they line up should they land some of their current targets? talkSPORT takes a look.Click the right arrow above to see how Liverpool’s starting XI could look with Mario Gotze, Joel Matip and other possible signings… Left back: Jonas Hector Centre back: Joel Matip Central midfield: Emre Can Central midfield: James Milner Striker: Mario Gotzelast_img read more

Castro rues poor transition D, not his cold shooting, in loss to Globalport

first_imgLOOK: Venues for 2019 SEA Games Gavina blows his top after yet another Kia loss: ‘I’m tired of this’ UPLB exempted from SEA Games class suspension And these attempts were point blank floaters and layups that Castro, who finished with 15 points, usually makes.“Everybody has off nights but I think our mistake was our transition defense because once they get the ball we let them start running,” said Castro in Filipino.FEATURED STORIESSPORTSWATCH: Drones light up sky in final leg of SEA Games torch runSPORTSSEA Games: Philippines picks up 1st win in men’s water poloSPORTSMalditas save PH from shutoutThere were also some calls—or lack thereof—that seemed questionable for TNT but Casto would not comment on the officiating in the loss.“We can’t do anything about it, that was what the referees called so we just have to focus on the game next time around,” said Castro. LATEST STORIES View comments The KaTropa failed to stretch their winning streak to three with the loss as they dropped to 4-3. Kammuri turning to super typhoon less likely but possible — Pagasa MOST READ Catriona Gray spends Thanksgiving by preparing meals for people with illnessescenter_img PBA IMAGESANTIPOLO—–TNT point guard Jayson Castro had the coldest hands inside Ynares Sports Center in the KaTropa’s loss 119-112 loss to Globalport in the PBA Governors’ Cup.Castro went on a decent 5-of-12 clip from the field but missed multiple crucial shots in the fourth quarter when the KaTropa attempted to come back against the Batang Pier.ADVERTISEMENT Don’t miss out on the latest news and information. Typhoon Kammuri accelerates, gains strength en route to PH SEA Games in Calabarzon safe, secure – Solcom chief Brace for potentially devastating typhoon approaching PH – NDRRMC Read Next WATCH: Streetboys show off slick dance moves in Vhong Navarro’s wedding Sports venues to be ready in time for SEA Games PLAY LIST 00:59Sports venues to be ready in time for SEA Games01:27Filipino athletes get grand send-off ahead of SEA Games01:42Police: California school shooting took 16 seconds01:37Protesters burn down Iran consulate in Najaf01:47Panelo casts doubts on Robredo’s drug war ‘discoveries’01:29Police teams find crossbows, bows in HK university01:35Panelo suggests discounted SEA Games tickets for students02:49Robredo: True leaders perform well despite having ‘uninspiring’ boss02:42PH underwater hockey team aims to make waves in SEA Gameslast_img read more

Shoaib Akhtar gives coach Mickey Arthur eight-month deadline to improve Pakistan cricket

first_imgThe former fast bowler Shoaib Akhtar has given new coach Mickey Arthur eight months of deadline to improve Pakistan cricket or step down from the position if he fails in his assignment.However, Akhtar backed the appointment of Arthur as Pakistan’s coach and feels the Pakistan players will benefit from his past international experience with top teams.”But I think in eight months time we will know whether he will make a difference or go like others before him. Because in this period we play some very important series and either he will reshape the way we play our cricket or decide to bid goodbye,” Akhtar said.Akhtar also pointed out that coaching Pakistan is a tough job for any foreign coach because of different cricket culture in the country.’Disciplinarian and innovative'”Arthur has a reputation of being a disciplinarian and innovative coach and Pakistan cricket needs both but he has to decide when and where to draw the line,” Akhtar told a local television channel.Pakistan in the next eight months has full Test tours to England, New Zealand and Australia and also play the West Indies in a full series in the UAE.”Arthur has all the credentials to change the attitude and mindset of our players the way they play top cricket. So I am hopeful.”The fast bowler, whose colourful career included number of clashes with the establishment, said while he agreed that discipline was necessary in cricket teams but insisted players needed to be made to feel responsible for their actions as professional athletes.advertisement”Only a professional player can decide what is best for him. When I played there were some undisciplined characters in the team but on match days they only focused on cricket and when they went out to play they tried to give their best that is why our team in those days was so good,” he recalled.Referring to the decision by the new selection committee to drop batsmen Ahmed Shehzad and Umar Akmal from the training camp being set up for the coming tour to England, Akhtar said both players had brought this upon themselves.Players need to start performing “For a long time we have all backed them, appreciated and encouraged their undoubted talent but while others who started with them have moved on to become top performers for their country we are yet to see the best from them.”Akhtar said both needed to rethink their priorities and focus on their cricket and do justice to their talent. “I believe it is important for players to be relaxed on non-match days but what matters is you perform when required.”Asked about the accusations by Arthur in 2009 about having suspicions that the fifth and final one-day international that Pakistan played a fixed game against South Africa in Lahore in 2007, Akhtar said all this was rubbish.”I played in that match and I took four wickets. I was at the non-strikers end when our wickets fell. We just played badly under pressure. Because if there had been anything fishy going on believe me I would have dealt with such players myself,” Akhtar said.He said Arthur had clarified his stance and one needed to move on and not keep bringing up the issue now.Akhtar also criticized the month-long training camp being set up before the England tour and emphasis on fitness tests.”I don’t understand this practice because it is hot and every player has his own particular fitness issues and requirements. You cannot lay down same fitness standards for a batsman, spinner and fast bowler. It makes no sense to me.”I always had a knee problem but carried on for so long in international cricket because I knew what I had to do.”(with PTI inputs)last_img read more